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ACT may refer to:

Most commonly:

• Australian Capital Territory, the capital territory of the Commonwealth of Australia
• ACT (examination), a college entrance exam in the United States

In business:

• Advanced Cell Technology, a biotech corporation involved in researching and developing cloning and stem cell technologies.
• Ada Core Technologies, a software company that develops an Ada compiler, tools and libraries
• Applied Computer Techniques, a computer services company, renamed Apricot Computers in 1985
• Aviation Composite Technology, an aircraft manufacturer in the Philippines
• ACT (Nasdaq), Automated Confirmation of Transactions, a trade reporting and clearing system
• Asia Container Terminals Ltd.

Organizations:

• Academy of Clinical Thyroidologists
• Association for Competitive Technology, a pro-Microsoft lobby group
• Association of Cinematograph Technicians
• Association of Corporate Treasurers
• Action for Children's Television, an organization dedicated to improvements in children's television
• ACT New Zealand, a political party and movement in New Zealand
• Alliance of Concerned Teachers, an alliance of teachers, educators and teacher organizations in the Philippines
• America Coming Together, a United States political group
• AIDS Committee of Toronto
• Association for Citizenship Teaching, a professional subject association for teachers of citizenship

In government:

• Allied Command Transformation, a NATO strategic command
• Advanced Concepts Team, a research team of the European Space Agency

In science and technology:

• ACT (audio format) used in some Chinese MP4/MTV players
• ACT (cognitive model), a symbolic cognitive architecture
• ACT!, contact management software
• Annual Change Traffic, the fraction of a software product's source code that changes in a year
• Apple Certified Trainer
• Astrographic Catalog/Tycho, a star catalogue
• Atacama Cosmology Telescope, a telescope under construction in Chile

In medicine:

• Artemisinin-based Combination Therapy, a treatment for malaria
• Assertive Community Treatment, a system for treating mental illness
• Acceptance and Commitment Therapy, a type of cognitive-behavioral therapy
• Autologous cartilage transplantation, a cartilage replacement operation

In entertainment:

• A.C.T, a progressive rock band from Sweden
• American Conservatory Theater, in San Francisco, California
• ACT Theatre, in Seattle, Washington

Other:

• Ascot railway station, a railway station in the United Kingdom with the National Rail code "ACT"
• Waco Regional Airport, an airport in Waco, Texas, United States, with the IATA airport code "ACT"
• ACT can be abbreviation for activity